Part:BBa_K1510930:Design

Constitutive promoter and RBS region for Streptococcus mutans transcription.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The promoter and RBS region would only be in need in the functional measurement stage of our project so that we could test whether the S.mutans with luxI gene below our constitutive promoter and RBS region could normally express the quorum sensing signal AHL or not. Whereas, in the working circuit, this promoter and RBS region won't be added because the luxI gene and its terminator are ligated to a complete sequence with CSP-sensing promoter region.

Source

The constitutive promoter and RBS region is obtained from the genome of Streptococcus mutans by self-designed PCr primers.